Clinical microcirculatory exploration


Our lab has been dedicated to the development and the evaluation of devices exploring skin microvasculature, in collaboration with academic and industrial partners.

We have worked on the reproducibility of several non-invasive techniques, including laser Doppler and the recently available laser speckle contrast imaging.


Equipement/ Material

  • Laser speckle contrast imager (Perimed PeriScan)
  • Laser-Doppler flowmeters (Perimed PF5000)
  • Laser-Doppler imager (Perimed PIM3)
  • Videocapillaroscopy (Xport Technologies)
  • Reactivity tests:
    • Cold challenges (Perimed, Moor, prototypes)
    • Post-occlusive reactive hyperemia
    • Iontophoresis
    • Skin microdialysis



  • Cracowski JL, Gaillard-Bigot F, Cracowski C, Roustit M, Millet C. Skin microdialysis coupled with Laser Speckle Contrast Imaging to assess microvascular reactivity. Microvasc Res. 2011 Nov;82(3):333-8.
  • Roustit M, Cracowski JL. Non-invasive assessment of skin microvascular function in humans: an insight into methods. Microcirculation 2011 Aug 28. doi: 10.1111/j.1549-8719.2011.00129.x.
  • Millet C, Roustit M, Blaise S, Cracowski JL. Comparison between laser speckle contrast imaging and laser Doppler imaging to assess skin blood flow in humans. Microvasc Res. 2011 Sep;82(2):147-51.
  • Roustit M, Millet C, Blaise S, Dufournet B, Cracowski JL. Excellent reproducibility of laser speckle contrast imaging to assess skin microvascular reactivity. Microvasc Res 2010 Dec;80(3):505-11.
  • Roustit M, Blaise S, Millet C, Cracowski JL. Reproducibility and methodological issues of skin post-occlusive and thermal hyperemia assessed by single-point laser Doppler flowmetry. Microvasc Res. 2010 Mar;79(2):102-108.
  • Roustit M, Maggi F, Isnard S, Hellmann M, Bakken B, Cracowski J-L. Reproducibility of a local cooling test to assess microvascular function in human skin. Microvasc Res 2010 January; 79(1):34-39.
  • Cracowski JL, Minson CT, Salvat-Melis M, Halliwill JR. Methodological issues in the assessment of skin microvascular endothelial function in humans. Trends in Pharmacological Sciences. 2006 27: 503-508